FABP Rapid Test strips (Fatty Acid Binding Protein)
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fatty acid binding
fatty acid binding protein
hFABP Rapid Test Card is an immunochromatography based one step in vitro test. It is designed for qualitative determination of Fatty Acid Binding Protein(FABP) in human serum or plasma specimens as an aid in the diagnosis of myocardial infarction.
Fatty Acid Binding Proteins (FABP) are tissue specific intracellular molecules of about 15 kD, they are a class of cytoplasmic proteins that bind long chain fatty acid and play an important role in the intracellular utilization of fatty acid.
Different FABP have been detected and these include heart FABP, liver FABP and intestinal FABP, etc. Human cardiac muscle has high content of FABP(10-20% of cytoplasmic proteins) . Heart FABP(hFABP) is a sensitive biomarker of myocardial necrosis that can be used to confirm or exclude a diagnosis a diagnosis of acute myocardial infarction(AMI) and for monitoring of a recurrent infarction. In AMI, hFABP is rapidly released from damaged cardiomyocytes into the circulation due to its small size. hFABP levels are significantly elevated above their threshold level within 3 hours after AMI and subsequently return to normal values in 12 to 24 hours.
More recently, hFABP has been identified as a potential serum biomarker for stroke that is superior to either neuron specific enolase or S100B. The normal levels of hFABP range from 1.6 ng/ml to an upper level of 19 ng/ml in various studies of cardiovascular disease. HFABP increases slightly with age. The sensitivity of this rapid is 8ng/ml.
The hFABP Rapid Test Card is a qualitative, solid phase, two-site sandwich immunoassay for the detection of human heart FABP in serum. The membrane is pre-coated with anti-hFABP antibodies on the test band region and anti-mouse antibodies on the control band region. During testing, the serum sample reacts with the colored conjugate (mouse anti-hFABP antibody colloidal gold conjugate) which has been pre-coated on the test strip. The mixture migrates upward on the membrane chromatographically by capillary action to react with anti-hFABP antibodies on the membrane and generate a red band. Presence of the red band indicates a positive result, while its absence indicates a negative result. Regardless of the presence of hFABP, as the mixture continues to migrate across the membrane to the immobilized goat anti-mouse region, a red band at the control band region will always appear. The presence of this red band serves as verification for sufficient sample volume and proper flow and as a control for the reagents.