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One-Step FOB Test is a rapid, visual immunochromatographic test for the qualitative detection of human blood hemoglobin in fecal samples. This test is intended as an aid in the diagnosis of lower gastrointestinal (g. i. ) disorders. The test is recommended for professional use.
 Review Specimen Collection instructions. Test device, patients samples, and controls should be brought to room temperature (20-300) prior to testing. Do not open pouches until ready to perform the assay.
 Remove the test device from the foil pouch and use it as soon as possible. Best results will be obtained if the assay is performed within one hour. Remove the test device from its protective pouch (bring the device to room temperature before opening the pouch to avoid condensation of moisture on the membrane) . Label the device with patient or control number.
 Shake the collection tube thoroughly to ensure proper mixing of the fecal sample with the extraction solution.
 Using a piece of tissue paper, break the tip of the collection tube using a twisting motion. Hold the collection tube vertically and dispense 3 drops of solution into the sample well of the test device.
 Observe the result in 5 minutes. Strong positive results may be observed sooner. Do not interpret after 8 minutes.
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