Sell Ni-IDA Resin

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Ni-IDA Resin

High-Affinity Ni-IDA Resin (Cat. No. RG00223, 50 ml of 50% slurry) is an agarose resin (4% cross-linked) covalently coupled to a tridentate chelating agent (iminodiacetic acid, called IDA) that binds Ni2+ ions at three coordination sites, It is particularly useful for the high-affinity purification of 6xHis-tagged recombinant proteins from any recombinant expression system, including bacterial, yeast, insect cell, and mammalian cells. These His-tagged recombinant proteins can be purified under either native or denaturing conditions. Proteins bound to the resin are then eluted with either low-pH buffer or imidazole solution or even with histidine solution.
Key Features:
 High binding capacity: The kit can handle over 10 mg 6xHis-tagged protein/ml (CV) .
 Simple purification procedure under both native and denaturing conditions.
 Broad pH stability: The resin works well between pH 3 and pH 13, and can manage pH 2 and pH14 for short periods.
 Resilient: If properly regenerated, the resin can be reused multiple times.


1. Purification of polyhistidine-tagged proteins under native conditions.
2. Purification of polyhistidine-tagged proteins from E. coli under denaturing conditions.


Store the product between 40C and 80C.
 For ambient pressure use only.
Cat. No. Name Size Price

RG00223 Ni-IDA Resin 25 ml $ 168.00

RG00223 Ni-IDA Resin 10 ml $ 80.00

RG00223 Ni-IDA Resin 500 ml $ 2480.00

RG0272 IminoBiotin Resin 5 ml $ 118.00
Brand Name
Supply Capacity
over 100 pcs Per Month)
Available Colors
Condition of Goods
Lead Time from Order to Shipment
within 30 days
Model Number
Minimum Order Quantity
1~5 pcs
Patents or Trademarks
Terms of Payment
100%T/T advance
Terms of Sale
FOB Shanghai