Sell Ni-IDA Resin

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Ni-IDA Resin
Description:

High-Affinity Ni-IDA Resin (Cat. No. RG00223, 50 ml of 50% slurry) is an agarose resin (4% cross-linked) covalently coupled to a tridentate chelating agent (iminodiacetic acid, called IDA) that binds Ni2+ ions at three coordination sites, It is particularly useful for the high-affinity purification of 6xHis-tagged recombinant proteins from any recombinant expression system, including bacterial, yeast, insect cell, and mammalian cells. These His-tagged recombinant proteins can be purified under either native or denaturing conditions. Proteins bound to the resin are then eluted with either low-pH buffer or imidazole solution or even with histidine solution.
Key Features:
 High binding capacity: The kit can handle over 10 mg 6xHis-tagged protein/ml (CV) .
 Simple purification procedure under both native and denaturing conditions.
 Broad pH stability: The resin works well between pH 3 and pH 13, and can manage pH 2 and pH14 for short periods.
 Resilient: If properly regenerated, the resin can be reused multiple times.

Applications:

1. Purification of polyhistidine-tagged proteins under native conditions.
2. Purification of polyhistidine-tagged proteins from E. coli under denaturing conditions.

Storage:

Store the product between 40C and 80C.
Note:
 For ambient pressure use only.
Cat. No. Name Size Price

RG00223 Ni-IDA Resin 25 ml $ 168.00


RG00223 Ni-IDA Resin 10 ml $ 80.00


RG00223 Ni-IDA Resin 500 ml $ 2480.00


RG0272 IminoBiotin Resin 5 ml $ 118.00
Brand Name
realgene
Supply Capacity
over 100 pcs Per Month)
Certifications
ISO9001Certificate
Available Colors
white
Condition of Goods
New
Lead Time from Order to Shipment
within 30 days
Model Number
RG00223
Minimum Order Quantity
1~5 pcs
Patents or Trademarks
realgene
Terms of Payment
100%T/T advance
Terms of Sale
FOB Shanghai
Weight
50~100g