Protein A Resin
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Cat. No. : RGM00089
Size: 1 mg in 1 ml of storage buffer
Molar Ratio of HRP/Protein A: 2:1.
Concentration: 1 mg /ml in PBS (pH 7.4) containing 10 mg /ml BSA and 0.01% Thimerosal.
Description:Staphylococcal Protein A binds strongly and specifically to the Fc region of immunoglobulin molecules, especially IgG of many species such as Human, Rabbit, Pig, etc. . The product contains 5 high affinity binding sites that are capable of interacting with the Fc region of IgG. HRP-Protein A is produced by covalently linking HRP to Protein A, which can be used in immunodetection such as immunoblotting (western or dot blot) , ELISA, and immunohistochemistry.
Storage: Store at 4 centi-degree for short term (one-month ) . Aliquot and store at -20 centi-degree for longer storage. Avoid repeated freeze/thaw cycles. If slight precipitation occurs upon prolonged storage , clarify the solution by centrifugation before use.
Working Dilution : Working concentrations for specific applications should be detemined by titration assay. Appropriate concentrations will be affected by several factors including primary antibody, antigen amount, sensitivity of detection method, temperature, and incubation time, etc. The following dilution ranges are just a guideline:
Dot blot: 1:5,000~1:20,000
Western blot: 1:5,000~1:20,000
Immunohistochemistry: 1:500~ 1:2,5 00