Saliva Alcohol Test Strip
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saliva alcohol test
saliva alcohol test strip
Saliva Alcohol Test is intended for use as a rapid method to detect the presence of alcohol in saliva for blood alcohol concentration (BAC) greater than 0.02%. It has been published that the concentration of alcohol in saliva is almost equal to that in blood.4
The test is intended for the semi-quantitation of ethyl alcohol in human saliva. To confirm the concentration of positive specimens, an alternate, non-enzymatic technology such as gas chromatography should be used.
EXPLANATION OF THE TEST
Alcohol intoxication can lead to loss of alertness, coma, death and as well as birth defects. The BAC at which a person becomes impaired is variable. The United States Department of Transportation (DOT) has established a BAC of 0.02% (0.02g/dL) as the cut-off level at which an individual is considered positive for the presence of alcohol.1-3
Determination of ethyl alcohol in blood and saliva is commonly used for measuring legal impairment, alcohol poisoning, etc. Gas chromatography techniques and enzymatic methods are commercially available for the determination of ethyl alcohol in human fluids. Saliva Alcohol Test is designed as the screen tool to rapidly determine if the BAC level is higher than 0.02% by testing saliva specimen.
PRINCIPLE OF THE PROCEDURE Saliva Alcohol Test is based on the high specifity of alcohol oxidase (ALOx) for ethyl alcohol in the presence of peroxidase and enzyme substrate such as tetramethylbenzidine (TMB) as shown in the following:
The distinct color on reactive pad could be observed in less than 20 seconds after the tip was contacted with saliva samples with the ethyl alcohol concentration greater than 0.02%. It should be pointed out that other alcohols such as methyl, propanyl and allyl alcohol would develop the similar color on the reactive pad. However, these alcohols are not normally present in saliva.